human liver tumor cell lines hepg2  (ATCC)

Journal: Open Medicine

Article Title: Superenhancer–transcription factor regulatory network in malignant tumors

doi: 10.1515/med-2021-0326

Figure Lengend Snippet: Core transcription factors in eight malignant tumor cell lines

Article Snippet: Human gastric malignant tumor cell line MKN45, human renal malignant tumor cell line 786-M1A, human esophageal squamous cell line KYSE140, human colorectal malignant tumor cell line HCT116, human bladder malignant tumor cell line T24, and human breast malignant tumor cell line 76NF2V, small cell lung malignant tumor cell line COR-L311, and human liver malignant tumor cell line HepG2 were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques:

Journal:

Article Title: Metabolites of the carcinogen 2-amino-alpha-carboline formed in male Sprague-Dawley rats in vivo , and in rat hepatocyte and human HepG2 cell incubates

doi: 10.1021/tx600303d

Figure Lengend Snippet: Quantitation of AaC metabolites present in rat bile, rat urine, rat hepatocyte incubates, and human HepG2 cell incubates.

Article Snippet: Human HepG2 liver tumor cells were obtained from ATCC and were cultured according to ATCC guidelines (HepG2/C3A, ATCC #CRL-10741).

Techniques: Quantitation Assay

Journal:

Article Title: Metabolites of the carcinogen 2-amino-alpha-carboline formed in male Sprague-Dawley rats in vivo , and in rat hepatocyte and human HepG2 cell incubates

doi: 10.1021/tx600303d

Figure Lengend Snippet: Flow scintillation radio-chromatograms used for quantitation of [3,4,5-3H]AaC metabolites in (A) HepG2 cell incubates and (B) rat hepatocyte incubates. Metabolite identifications were made by LC/MS/MS, NMR, and UV analyses.

Article Snippet: Human HepG2 liver tumor cells were obtained from ATCC and were cultured according to ATCC guidelines (HepG2/C3A, ATCC #CRL-10741).

Techniques: Quantitation Assay, Liquid Chromatography with Mass Spectroscopy

Journal:

Article Title: Metabolites of the carcinogen 2-amino-alpha-carboline formed in male Sprague-Dawley rats in vivo , and in rat hepatocyte and human HepG2 cell incubates

doi: 10.1021/tx600303d

Figure Lengend Snippet: Overlay of six multiple reaction monitoring (MRM) extracted ion chromatograms (XIC) used for structure identification of [3,4,5-3H]AaC metabolites in (A) HepG2 cell incubates, and (B) rat hepatocyte incubates. Blue color indicates MRM XIC from 280/200 m/z pair, red indicates 322/242 m/z pair, blue-green indicates 226/184 m/z pair, pink indicates 360/184 m/z pair, gray indicates 376/200 m/z pair, and light blue indicates 418/242 m/z pair.

Article Snippet: Human HepG2 liver tumor cells were obtained from ATCC and were cultured according to ATCC guidelines (HepG2/C3A, ATCC #CRL-10741).

Techniques: Targeted Proteomics

Journal:

Article Title: Metabolites of the carcinogen 2-amino-alpha-carboline formed in male Sprague-Dawley rats in vivo , and in rat hepatocyte and human HepG2 cell incubates

doi: 10.1021/tx600303d

Figure Lengend Snippet: NMR spectra of (A) AaC and (B) M10 purified from human HepG2 incubations. M10 corresponds to metabolite in Figure 3 eluting at 28 min, and corresponds to metabolite in Figure 4 with MRM pair (280/200) eluting at 25.5 min. Aromatic proton resonance region (6.3-8.2 ppm) is shown.

Article Snippet: Human HepG2 liver tumor cells were obtained from ATCC and were cultured according to ATCC guidelines (HepG2/C3A, ATCC #CRL-10741).

Techniques: Purification